Putative endolysin

Author: alpd

August undefined, 2024

WebApr 11, 2024 · Aiming at utilizing a phage endolysin as a therapeutic alternative to antibiotics, we surveyed the genome and bacteriophage sequences of C. perfringens. A … WebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum …

A Computational Method for the Identification of Endolysins and ...

WebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This paper characterizes a Salmonella phage endolysin (Lys68) and demonstrates its potential antimicrobial effectiveness when combined with organic acids towards Gram-negative … WebEndolysin is the term for muralytic enzymes that degrade the cell wall; endolysins accumulate in the cytosol fully folded during the vegetative cycle. ... Holins constitute one of the most diverse functional groups, with >100 known or putative holin sequences, which form >30 ortholog groups. Publication types Research Support, Non-U ... gregor the overlander book 1

Expanding the Database of Signal-Anchor-Release Domain

WebClostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential … WebAug 6, 2016 · Endolysin from Staphylococcus aureus phage SA97 (LysSA97) was cloned and investigated. LysSA97 specifically lyse the staphylococcal strains and effectively disrupted staphylococcal biofilms. Bioinformatic analysis of LysSA97 revealed a novel putative cell wall binding domain (CBD) as well as two enzymatically active domains … WebMar 1, 2024 · that a 36.1-kbp putative phage remnant region exists only on the genome of C. perfringens st13 and is absent from the genomes of the other sequenced C. perfringens strains (Figure S2). A gene designated psa (gene ID; CPE1138), encoding a putative endolysin, was detected at one end of this region; a putative holin gene is also present in … gregor the overlander book 2

Identification and characterization of an endolysin encoded by ...

Characterization and determination of holin protein of

WebJul 21, 2024 · In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. … WebMar 23, 2024 · To enable functional and structural studies of the putative Enc34 endolysin, the coding sequence of the ORF39 protein with a cleavable N-terminal hexahistidine-tag was cloned and expressed in ... gregor the overlander authorWebApr 12, 2011 · We found that an endolysin gene (psm) in phiSM101, an episomal phage of enterotoxigenic C. perfringens type A strain, SM101 (Myers et al. 2006), encodes a … gregor the overlander book 2 read online free

"WebMay 7, 2024 · Dataset. We evaluated 2628 putative endolysin genes found in 183,298 genomic sequences of uncultured viral genomes previously identified by Fernández-Ruiz … " - Putative endolysin

Putative endolysin

Expanding the Database of Signal-Anchor-Release Domain

WebMay 11, 2024 · The putative endolysin gene was identiﬁed from the CPS2 genome and designated as LysCPS2. Pfam and Conserved Domain Database analysis revealed that LysCPS2 is a putative WebA Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm Hiroshi Sekiya et al. Antibiotics, 2024 Ts2631 Endolysin from the Extremophilic Thermus scotoductus Bacteriophage vB_Tsc2631 as an Antimicrobial Agent against Gram-Negative Multidrug …

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WebApr 10, 2024 · As a result, any dynamic endolysin-based infection power merchants must combine outer-membrane-permeabilizing marketers after remaining ... Wang G et al (2024) Biological properties and genomics analysis of vB_KpnS_GH-K3, a Klebsiella phage with a putative depolymerase-like protein. Virus Genes 55:696–706. Article CAS PubMed ... WebNov 22, 2024 · In the present work, a putative endolysin (AbLys1) was identified and characterized. Ab Lys1 was evaluated for its antimicrobial activity against a clinically isolated A. baumannii strain. The work aimed at the discovery of new antimicrobial agents towards A. baumannii cells as an alternative non-antibiotic strategy to combat this …

WebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This …

WebThe 723 putative endolysins ranged from 72 to 578 residues in length, using 24 different catalytic domains and 18 different cell wall-binding domains organized with 89 different … WebNov 12, 2024 · In this study, the potential role of the VL1_T00003720 (denominated as Vl3720) gene, encoding a putative endolysin-like protein, has been investigated in the …

WebApr 11, 2024 · Aiming at utilizing a phage endolysin as a therapeutic alternative to antibiotics, we surveyed the genome and bacteriophage sequences of C. perfringens. A phiSM101 muramidase gene (psm) revealed by this study can be assumed to encode an N-acetylmuramidase, since the N-terminal catalytic domain deduced from the gene shows …

WebMar 28, 2012 · Identification of putative cell wall binding domains. With only a single exception (Myrna gp243), the putative endolysin catalytic domain is flanked on its C … gregor the overlander book 6WebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, … gregor the overlander book 5 pdfWebFeb 15, 2024 · The gene (GenBank accession no. OL415943) encoding putative endolysin from bacteriophage PBEC131 was cloned into pET21a+ (Novagen, United States) using … gregor the overlander book 4 read online freeWebMethods: We propose a computational method for the prediction of endolysin and autolysin. First, a data set containing 27 endolysins and 41 autolysins is built. Then the protein is … gregor the overlander book 5WebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, encodes the putative endolysin.ORF43, which is 429 bp long and upstream of the lySMP gene, is predicted to encode a putative holin protein, designated HolSMP (Figure … gregor the overlander book 7WebPreviously, bioinformatic approaches resulted in a database of 66 SAR endolysins. This manuscript almost doubles the list with 53 additional SAR endolysin candidates. Forty … gregor the overlander chapter 14WebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the ... gregor the overlander book summary