WebUnder sterile operating conditions, a vitreous fluid (0.1–0.2 ml) was obtained by pars plana vitrectomy procedure. The samples were processed for conventional microbiology … Web4 nov. 2015 · Each PCR reaction mixture (25 µ l) contained 20 ng genomic DNA, 2X MightyAmp Buffer ver. 2 (Takara), 0.25 µ M of each primer, and 1.25 units of MightyAmp DNA Polymerase (Takara). Each PCR amplification and preparation of amplicon pool were performed as described by Takahashi et al ( 30 ).
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WebMightyAmp DNA Polymerase is designed for hot start PCR. It includes monoclonal antibodies to inhibit polymerase activity at temperatures up to 98℃, thereby preventing … Webusing MightyAmp DNA Polymerase (Takara). The sequences of primers used for genomic PCR were AGCACCGTCTTTGCAAGTCA (forward) and TCCAGA GCCCACTTGCTTAAC (reverse). The annealing tempera-ture was 58 °C, the elongation time was 30 s, and the cycle number was 30. The PCR products were annealed with themselves, and then 0.5 … Web12 apr. 2024 · MightyAmp™ DNA Polymerase Ver.3酶试剂盒Takara; RatioWorks pH荧光探针PDMPO 货号21204-AAT Bioquest荧光染料; BD培养基 211720 500 g EA BBL Thioglycollate Medium without Indicator 135C 不含指示剂硫乙醇酸盐培养基; V20 容量法 中文卡式水分仪-卡尔费休水分仪-梅特勒托利多 ryan adams music videos