WebApr 13, 2024 · BY4742 yeast requires Cbp3 and Cbp6 for efficient COB mRNA translation, whereas the D273-10b strain synthesizes Cytb at wildtype levels in the absence of Cbp3 and Cbp6. Steady-state levels of Cyt b are close to wildtype in mutant D273-10b cells, and Cyt b forms non-functional, supercomplex-like species with cytochrome c oxidase, in … Web摘要 以酿酒酵母Saccharomyces cerevisiae BY4742为宿主茵,利用DNA组装(DNA assemble)技术,向宿主茵导入了β-胡萝卜素合成途径,表达了源自Xanthophyllomyces dend... 展开更多 By the DNA assembly method, the β-carotene biosynthesis pathway was induced into the host strain, Saccharo- myces cerevisiae BY4742, which successfully expressed …
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WebViable bacteria decreased over 72 h in corn mash fermentation. In the negative control, yeast-only sample, no measurable bacterial level (> 3-log CFU/mL) was detected from 0 h–72 h ().Yeast samples with positive controls challenged with L. fermentum infection showed a steady log CFU/mL increase over a period of 48 h from 7.6–9.4 log CFU/mL … WebAug 29, 2024 · We transformed BY4741 and BY4742 cells with the Cas9/gRNA plasmid targeting the 5 Ty3 LTRs and recovered a total of 211 and 159 transformants, respectively. We PFGE karyotyped 69 transformants (37 BY4741 and 32 BY4742) out of which 30 showed clear chromosomal rearrangements on the gels, representing 18 different … hospitalist case studies
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WebJul 28, 2024 · The MATa haploid strain of S. cerevisiae BY4742 (Thermo Fisher Scientific, Waltham, MA, USA) was used as a host for promoter engineering and combinatorial … Webthe BY4742 strains, His+ frequencies peaked after two or three subcultures and then noticeably declined (Fig. 1C, left panel). For the GRF167 strains, the highest His+ fre-quencies were obtained after four to five subcultures, but they then decreased only modestly (Fig. 1C, right panel). The median peak His+ frequencies for the BY4742-de- hospitalist cme 2020